- Research Professor, University of Notre Dame
- Associate Director, W.M. Keck Center for Transgene Research
- Research Associate Professor, University of Notre Dame
- Assistant Staff, Cleveland Clinic Research Foundation
- Senior Scientist and Director of Cardiovascular Research, American Biogenetic Sciences, Inc.
- Postdoctoral Researcher, University of Notre Dame and Scripps Research Institute
- Ph.D. in Biochemistry, University of Notre Dame
- B.A. in Biology and Chemistry, Rosary College
- Fellow, Council on Atherosclerosis, Thrombosis, and Vascular Biology (American Heart Association)
- James A. Shannon NIH Director's Award
- John Hickam Fellow, American Heart Association (Indiana Affiliate)
The fibrinolytic system is composed of the zymogen, plasminogen (Pg); its active enzyme (Pm); the plasminogen activators, tissue plasminogen activator (tPA) and urokinase (uPA); and relevant inhibitors plasminogen activator inhibitor-1 (PAI-1) and a2-antiplasmin. This system has been implicated in playing a pivotal role in numerous physiological processes. Due to the ability of plasmin to degrade fibrin, the fibrinolytic system plays an essential role in the prevention of thrombosis and maintenance of vascular patency. The ability of plasmin to directly degrade matrix protein, to activate other matrix degrading proteases and the existence of cellular receptors for components of the fibrinolytic system also implicates this pathway in localized proteolytic processes involved in normal cell migration, tissue remodeling, wound healing and angiogenesis. In addition, it's believed that the fibrinolytic system is involved in pathological processes where uncontrolled expression of proteolytic activity occurs, viz., tumor invasion and metastasis. However, much of the evidence for these diverse roles is surmised from in vitro studies and lack firm biological confirmation. Studies utilizing mice deficient for components of this pathway already have begun to challenge a number of the perceived roles of the fibrinolytic system. In addition, the lack of a more severe thrombotic phenotype and the occurrence of delayed clot lysis in mice deficient for Pg (PG-/-), would appear to support involvement of nonplasmin mediated fibrinolytic processes for maintaining some degree of vascular patency and most probably survival in these deficient mice, possibly due to leukocyte elastases.Utilizing mice deficient for components of the fibrinolytic system, our laboratory is currently testing hypothesized functions of this pathway when physiologically challenged. Specifically, we are assessing its' role in inflammation and diseases associated with inflammation, viz., asthma, atherosclerosis, pulmonary fibrosis as well as other physiological and pathophysiological processes in which cell migration is an essential event, viz., tumor growth, metastasis and angiogenesis. Additionally, we are isolating primary arterial and venous endothelial cells from these gene deficient mice in order to determine altered endothelial cell functions that may contribute to changes in angiogenesis.
- Yuan, Y.; Zajicek, J.; Qiu, C.; Chandrahas, V.; Lee, S. W.; Ploplis, V. A.; Castellino, F. J. "Conformationally organized lysine isosteres in Streptococcus pyogenes M protein mediate direct high-affinity binding to human plasminogen." J. Biol. Chem. 2017, 292, 15016-15027.
- Bao, Y.; Li, Y.; Liang, Z.; Agrahari, G.; Lee, S. W.; Ploplis, V. A.; Castellino, F. J. "Comparative pathogenomic characterization of a non-invasive serotype M71 strain Streptococcus pyogenes NS53 reveals incongruent phenotypic implications from distinct genotypic markers." Pathog. Dis. 2017, 75, ftx056.
- Glinton, K.; Beck, J.; Liang, Z.; Qiu, C.; Lee, S. W.; Ploplis, V. A.; Castellino, F. J. "Variable region in streptococcal M-proteins provides stable binding with host fibrinogen for plasminogen-mediated bacterial invasion." J. Biol. Chem. 2017, 292, 6775-6785.
- Gupta, K. K.; Donahue, D. L.; Sandoval-Cooper, M. J.; Castellino, F. J.; Ploplis, V. A. "Plasminogen Activator Inhibitor-1 Protects Mice Against Cardiac Fibrosis by Inhibiting Urokinase-type Plasminogen Activator-mediated Plasminogen Activation." Sci. Rep. 2017, 7, 365.
- Walsh, M.; Shreve, J.; Thomas, S.; Moore, E.; Moore, H.; Hake, D.; Pohlman, T.; Davis, P.; Ploplis, V.; Piscoya, A.; Wegner, J.; Bryant, J.; Crepinsek, A.; Lantry, J.; Sheppard, F.; Castellino, F. "Fibrinolysis in Trauma: "Myth," "Reality," or "Something in Between"." Semin. Thromb. Hemost. 2017, 43, 200-212.
- Bao, Y.; Shapiro, B. J.; Lee, S. W.; Ploplis, V. A.; Castellino, F. J. "Phenotypic differentiation of Streptococcus pyogenes populations is induced by recombination-driven gene-specific sweeps." Sci. Rep. 2016, 6, 36644.