Department of Chemistry & Biochemistry

Biography

2018-present

Director, Magnetic Resonance Research Center, University of Notre Dame

2018-present

Research Associate Professor, University of Notre Dame

2011-2018

Assistant Professor of Chemistry, Marquette University

2006-2011

Assistant Professor of Biochemistry, Medical College of Wisconsin

2001-2006

Postdoctoral Fellow, Yale University

2000-2001

Postdoctoral Fellow, UT Southwestern Medical Center - Dallas

1999-2000

Postdoctoral Scientist, Institute of Protein Research, Russia

1999

Ph.D. in Chemistry, Engelhardt Institute of Molecular Biology, Russia

1995

M.Sci. in Molecular Biology, Moscow State University, Russia

1993

B.Sci. in Chemistry, Krasnoyarsk State University, Russia

Selected Awards

2012, 2014

Summer Faculty Fellowship, Marquette University

2001

Anderson Postdoctoral Fellowship, Yale University

1999

MicroCal Young Scientist Award

Research Interests

Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful method to interrogate small and large molecules at the atomic level. In chemistry and biochemistry, NMR spectroscopy allows one to determine structure and conformation of molecules as well as to monitor their dynamic changes in the course of chemical reactions. NMR signals are also sensitive to subtle interactions between molecules, providing a unique window into the molecular interaction mechanisms. Biological polymers such as proteins and nucleic acids often undergo complex multi-step rearrangements between different conformations to accommodate their ligands—readily followed by the NMR spectroscopy to reveal their functional significance.

The Magnetic Resonance Research Center is a state-of-the-art NMR facility serving inorganic, organic, and biological chemists and material scientists at the University of Notre Dame and beyond. Seven NMR instruments ranging from 300 to 800 MHz are available for use by students, postdocs, and faculty for solution and solid-state NMR experiments. The Center provides individualized user training and supports continuous development of advanced NMR pulse programs and software for NMR data analysis to facilitate competitive cutting-edge research.

Recent Publications

• Chen, Y. S.; Kozlov, G.; Fakih, R.; Yang, M.; Zhang, Z. D.; Kovrigin, E. L. and Gehring, K. "Mg2+-ATP Sensing in CNNM, a Putative Magnesium Transporter" 2020 Structure, 28 (3), pp.324. DOI: 10.1016/j.str.2019.11.016.
• Feng, C.; Kovrigin, E. L. and Post, C. B. "NmrLineGuru: Standalone and User-Friendly GUIs for Fast 1D NMR Lineshape Simulation and Analysis of Multi-State Equilibrium Binding Models" 2019 Scientific Reports, 9, 16023. DOI: 10.1038/s41598-019-52451-8.
• McShan, A. C.; Natarajan, K.; Kumirov, V. K.; Flores-Solis, D.; Jiang, J.; Badstubner, M.; Toor, J. S.; Bagshaw, C. R.; Kovrigin, E. L.; Margulies, D. H. and Sgourakis, N. G. "Peptide Exchange on MHC-I by TAPBPR is Driven by a Negative Allostery Release Cycle" 2018 Nature Chemical Biology, 14 (8), pp.811. DOI: 10.1038/s41589-018-0096-2.
• Shishina, A. K.; Kovrigina, E. A.; Galiakhmetov, A. R.; Rathore, R. and Kovrigin, E. L. "Study of Forster Resonance Energy Transfer to Lipid Domain Markers Ascertains Partitioning of Semisynthetic Lipidated N-Ras in Lipid Raft Nanodomains" 2018 Biochemistry, 57 (5), pp.872-881. DOI: 10.1021/acs.biochem.7b01181.
• Galiakhmetov, A. R.; Kovrigina, E. A.; Xia, C. W.; Kim, J. and Kovrigin, E. L. "Application of Methyl-TROSY to a Large Paramagnetic Membrane Protein without Perdeuteration: C-13-MMTS-Labeled NADPH-Cytochrome P450 Oxidoreductase" 2018 Journal of Biomolecular NMR, 70 (1), pp.21-31. DOI: 10.1007/s10858-017-0152-3.
• Shinya, S.; Ghinet, M. G.; Brzezinski, R.; Furuita, K.; Kojima, C.; Shah, S.; Kovrigin, E. L. and Fukamizo, T. "NMR Line Shape Analysis of a Multi-State Ligand Binding Mechanism in Chitosanase" 2017 Journal of Biomolecular NMR, 67 (4), pp.309-319. DOI: 10.1007/s10858-017-0109-6.