Anthony Serianni

Research Interests

Professor Serianni's research focuses on the use of stable isotopically-labeled compounds to examine structure, conformation and reactivity of carbohydrates and nucleic acids in solution using modern multidimensional NMR methods and computational techniques. His group is interested in developing and improving chemical and chemi-enzymic methods to prepare carbohydrate-containing biomolecules containing one or more sites of isotopic enrichment, with ¹³C, ²H and ¹⁵N of primary concern. NMR studies of these labeled biomolecules permit a more detailed assessment of their 3-D structures in solution, information which is critical to understanding the fundamental factors controlling molecular recognition in biological processes.

Of current interest is the use of stable isotopes in the assessment of DNA and RNA structure in solution. Research is under way to: a) develop efficient chemical, chemi-enzymic, and/or biological methods to introduce isotopes selectively or uniformly into nucleosides and oligonucleotides, b) apply 2-D and 3-D NMR methods to decipher the NMR spectra of labeled oligonucleotides, and c) assess the merits of ¹³C-¹H and ¹³C-¹³C spin-coupling constants within the furanose rings of RNA and DNA as structural probes. NMR data are complemented and extended by ab initio molecular orbital calculations which can assess the energetics and structural features of furanose ring pseudorotation and predict ¹³C-¹H and ¹³C-¹³C spin-couplings for various pathways in these rings, and by X-ray crystallography. The long range objective is to apply this information to NMR solution studies of CCAAT-containing oligonucleotides involved in the NF-1 recognition site at the origin of replication of human adenovirus, and other biologically-important DNA and RNA oligomers.

Other work involves studies of carbohydrate metabolism via in vivo ¹³C and ¹⁹F NMR spectroscopy, and the development of automated devices for chemical and biological synthesis of labeled biomolecules.

Selected Publications

• Meredith, R. J.; Carmichael, I. and Serianni, A. S. "Geminal ¹³C-¹H NMR Spin-Coupling Constants in Furanose Rings: New Empirical Correlations with Conformation" 2025 ACS Omega, 10, pp.15309-15320. DOI: 10.1021/acsomega.4c11358.
• Yoon, M. K.; Shit, P.; Zhang, W. H.; Meredith, R. J.; Kang, H.; Carmichael, I. and Serianni, A. S. "^4h J _CHOCH Spin Coupling in a Lewis^X Trisaccharide as Evidence of Inter-Residue C-HO Hydrogen Bonding in Aqueous Solution" 2024 Journal of the American Chemical Society, 146 (45), pp.31264-31273. DOI: 10.1021/jacs.4c11948.
• Zhang, W. H.; Meredith, R. J.; Yoon, M. K.; Carmichael, I. and Serianni, A. S. "Context Effects on Human Milk Oligosaccharide Linkage Conformation and Dynamics Revealed by MA'AT Analysis" 2024 Biochemistry, 63 (21), pp.2729–2739. DOI: 10.1021/acs.biochem.4c00348.
• Zhang, W. H. and Serianni, A. S. "Action of Molybdate Anion on D-Glucosone: Catalytic Conversion to Aldonates Involving C1-C2 Transposition" 2024 ACS Omega, 9 (39), pp.40566–40572. DOI: 10.1021/acsomega.4c04139.
• Meredith, R. J.; Zhang, W. H.; Yoon, M. K.; Hu, X. S.; Carmichael, I. and Serianni, A. S. "MA'AT Analysis of the O-Glycosidic Linkages of Oligosaccharides using Nonconventional NMR J-Couplings: MA'AT and MD Models of Phi" 2024 RSC Advances, 14 (41), pp.30286–30294. DOI: 10.1039/d4ra06062h.
• Shit, P.; Tetrault, T.; Zhang, W. H.; Yoon, M. K.; Oliver, A. G. and Serianni, A. S. "Conformational Disorder in the Crystal Structure of Methyl 2-Acetamido-2-Deoxy-Β-D-Glucopyranosyl(1→4)-2-Acetamido-2-Deoxy-Β-D-Glucopyranoside (Methyl Β-Chitobioside) Methanol Monosolvate" 2024 Acta Crystallographica Section C-Structural Chemistry, 80 pp.331–336. DOI: 10.1107/S2053229624005199.