Department of Chemistry & Biochemistry

Biography

1999-present

Professor, University of Notre Dame

1988-1999

Associate Professor, University of Notre Dame

1982-1988

Assistant Professor, University of Notre Dame

1980-1982

Postdoctoral Research Associate, Cornell University

1980

Ph.D. in Biochemistry, Michigan State University

1975

B.S. in Biochemistry, Albright College

Selected Awards

2012

Fellow, Royal Society of Chemistry

2012

Fellow, American Chemical Society

2010

Fellow, American Association for the Advancement of Science

2006

Melville L. Wolfrom Award in Carbohydrate Chemistry, ACS Division of Carbohydrate Chemistry

2003

Kaneb Teaching Award

2001

John A. Boezi Memorial Alumnus Award, Michigan State University

1988

Horace S. Isbell Award in Carbohydrate Chemistry, ACS Division of Carbohydrate Chemistry

Research Interests

Professor Serianni's research focuses on the use of stable isotopically-labeled compounds to examine structure, conformation and reactivity of carbohydrates and nucleic acids in solution using modern multidimensional NMR methods and computational techniques. His group is interested in developing and improving chemical and chemi-enzymic methods to prepare carbohydrate-containing biomolecules containing one or more sites of isotopic enrichment, with ¹³C, ²H and ¹⁵N of primary concern. NMR studies of these labeled biomolecules permit a more detailed assessment of their 3-D structures in solution, information which is critical to understanding the fundamental factors controlling molecular recognition in biological processes.

Of current interest is the use of stable isotopes in the assessment of DNA and RNA structure in solution. Research is under way to: a) develop efficient chemical, chemi-enzymic, and/or biological methods to introduce isotopes selectively or uniformly into nucleosides and oligonucleotides, b) apply 2-D and 3-D NMR methods to decipher the NMR spectra of labeled oligonucleotides, and c) assess the merits of ¹³C-¹H and ¹³C-¹³C spin-coupling constants within the furanose rings of RNA and DNA as structural probes. NMR data are complemented and extended by ab initio molecular orbital calculations which can assess the energetics and structural features of furanose ring pseudorotation and predict ¹³C-¹H and ¹³C-¹³C spin-couplings for various pathways in these rings, and by X-ray crystallography. The long range objective is to apply this information to NMR solution studies of CCAAT-containing oligonucleotides involved in the NF-1 recognition site at the origin of replication of human adenovirus, and other biologically-important DNA and RNA oligomers.

Other work involves studies of carbohydrate metabolism via in vivo ¹³C and ¹⁹F NMR spectroscopy, and the development of automated devices for chemical and biological synthesis of labeled biomolecules.

Recent Publications

• Lin, J.; Oliver, A. G. and Serianni, A. S. "D-Mannosamine Hydrochloride (2-Amino-2-Deoxy-D-Mannose Hydrochloride): Ionic Hydrogen Bonding in Saccharides Involving Chloride and Aminium Ions" 2022 Acta Crystallographica Section C-Structural Chemistry, 78, pp.223. DOI: 10.1107/S2053229622002121.
• Meredith, R. J.; McGurn, M.; Euell, C.; Rutkowski, P.; Cook, E.; Carmichael, I. and Serianni, A. S. "MA'AT Analysis of Aldofuranosyl Rings: Unbiased Modeling of Conformational Equilibria and in Solution" 2022 Biochemistry, 61 (4), pp.239-251. DOI: 10.1021/acs.biochem.1c00630.
• Lin, J. Y.; Oliver, A. G. and Serianni, A. S. "Methyl Beta-Lactoside [Methyl Beta-D-Galactopyranosyl-(1 -> 4)-Beta-D-Glucopyranosude] Monohydrate: A Solvomorphism Study" 2021 Acta Crystallographica Section C-Structural Chemistry, 77, pp.668. DOI: 10.1107/S2053229621009499.
• Lin, J. Y.; Meredith, R. J.; Oliver, A. G.; Carmichael, I. and Serianni, A. S. "Two-Bond C-13-C-13 Spin-Coupling Constants in Saccharides: Dependencies on Exocyclic Hydroxyl Group Conformation" 2021 Physical Chemistry Chemical Physics, 23 (40), pp.22912-22922. DOI: 10.1039/d1cp03320d.
• Lin, J. Y.; Oliver, A. G.; Meredith, R. J.; Carmichael, I. and Serianni, A. S. "Isopropyl 3-Deoxy-Alpha-D-Ribo-Hexopyranoside (Isopropyl 3-Deoxy-Alpha-D-Glucopyranoside): Evaluating Trends in Structural Parameters" 2021 Acta Crystallographica Section C-Structural Chemistry, 77 pp.490. DOI: 10.1107/S205322962100749X.
• Meredith, R. J.; Woods, R. J.; Carmichael, I. and Serianni, A. S. "Reconciling MA ' AT and Molecular Dynamics Models of Linkage Conformation in Oligosaccharides" 2020 Physical Chemistry Chemical Physics, 22 (26), pp.14454-14457. DOI: 10.1039/d0cp01389g.