- 428 Stepan Chemistry
Notre Dame, IN 46556
- +1 574-631-7807
- Organic Chemistry
- Life Processes
Prospective Graduate Students
|1999-present||Professor, University of Notre Dame|
|1988-1999||Associate Professor, University of Notre Dame|
|1982-1988||Assistant Professor, University of Notre Dame|
|1980-1982||Postdoctoral Research Associate, Cornell University|
|1980||Ph.D. in Biochemistry, Michigan State University|
|1975||B.S. in Biochemistry, Albright College|
2012 Fellow, Royal Society of Chemistry
2012 Fellow, American Chemical Society
2010 Fellow, American Association for the Advancement of Science
2006 Melville L. Wolfrom Award in Carbohydrate Chemistry, ACS Division of Carbohydrate Chemistry
2003 Kaneb Teaching Award
2001 John A. Boezi Memorial Alumnus Award, Michigan State University
1988 Horace S. Isbell Award in Carbohydrate Chemistry, ACS Division of Carbohydrate Chemistry
Professor Serianni's research focuses on the use of stable isotopically-labeled compounds to examine structure, conformation and reactivity of carbohydrates and nucleic acids in solution using modern multidimensional NMR methods and computational techniques. His group is interested in developing and improving chemical and chemi-enzymic methods to prepare carbohydrate-containing biomolecules containing one or more sites of isotopic enrichment, with 13C, 2H and 15N of primary concern. NMR studies of these labeled biomolecules permit a more detailed assessment of their 3-D structures in solution, information which is critical to understanding the fundamental factors controlling molecular recognition in biological processes.
Of current interest is the use of stable isotopes in the assessment of DNA and RNA structure in solution. Research is under way to: a) develop efficient chemical, chemi-enzymic, and/or biological methods to introduce isotopes selectively or uniformly into nucleosides and oligonucleotides, b) apply 2-D and 3-D NMR methods to decipher the NMR spectra of labeled oligonucleotides, and c) assess the merits of 13C-1H and 13C-13C spin-coupling constants within the furanose rings of RNA and DNA as structural probes. NMR data are complemented and extended by ab initio molecular orbital calculations which can assess the energetics and structural features of furanose ring pseudorotation and predict 13C-1H and 13C-13C spin-couplings for various pathways in these rings, and by X-ray crystallography. The long range objective is to apply this information to NMR solution studies of CCAAT-containing oligonucleotides involved in the NF-1 recognition site at the origin of replication of human adenovirus, and other biologically-important DNA and RNA oligomers.
Other work involves studies of carbohydrate metabolism via in vivo 13C and 19F NMR spectroscopy, and the development of automated devices for chemical and biological synthesis of labeled biomolecules.
- Tetrault, T.; Meredith, R. J.; Yoon, M. K.; Canizares, C.; Oliver, A. G.; Carmichael, I. and Serianni, A. S. "One-Bond C-13-C-13 Spin-Coupling Constants in Saccharides: A Comparison of Experimental and Calculated Values by Density Functional Theory using Solid-State C-13 NMR and X-Ray Crystallography" 2023 Physical Chemistry Chemical Physics, 25 (23), pp.16048-16059. DOI: 10.1039/d2cp05363b.
- Tetrault, T.; Meredith, R. J.; Zhang, W. H.; Carmichael, I. and Serianni, A. S. "One-Bond 13C-1H and 13C-13C Spin-Coupling Constants as Constraints in MA'AT Analysis of Saccharide Conformation" 2022 Journal of Physical Chemistry B, 126 (46), pp.9506-9515. DOI: 10.1021/acs.jpcb.2c04986.
- Meredith, R. J.; Carmichael, I. and Serianni, A. S. "Nonconventional NMR Spin-Coupling Constants in Oligosaccharide Conformational Modeling: Structural Dependencies Determined from Density Functional Theory Calculations" 2022 ACS Omega, 7 (27), pp.23950-23966. DOI: 10.1021/acsomega.2c02793.
- Meredith, R. J.; Sernau, L. and Serianni, A. S. "MA'AT: A Web-Based Application to Determine Rotamer Population Distributions in Solution from Nuclear Magnetic Resonance Spin-Coupling Constants" 2022 Journal of Chemical Information and Modeling, 62 (13), pp.3135-3141. DOI: 10.1021/acs.jcim.1c01166.
- Meredith, R. J.; Tetrault, T.; Yoon, M. K.; Zhang, W. H.; Carmichael, I. and Serianni, A. S. "N-Acetyl Side-Chain Conformation in Saccharides: Solution Models obtained from MA ' AT Analysis" 2022 Journal of Organic Chemistry, 87 (13), pp.8368-8379. DOI: 10.1021/acs.joc.2c00189.
- Lin, J.; Oliver, A. G. and Serianni, A. S. "D-Mannosamine Hydrochloride (2-Amino-2-Deoxy-D-Mannose Hydrochloride): Ionic Hydrogen Bonding in Saccharides Involving Chloride and Aminium Ions" 2022 Acta Crystallographica Section C-Structural Chemistry, 78, pp.223. DOI: 10.1107/S2053229622002121.