Norman Dovichi

Norman Dovichi

Physical/Analytical Chemistry

Biography

2010-present
Grace Rupley Professor of Chemistry and Biochemistry, University of Notre Dame
2001-2010
Endowed Professor of Analytical Chemistry, University of Washington
1991-2000
Professor, University of Alberta
1986-1991
Associate Professor, University of Alberta
1982-1986
Assistant Professor, University of Wyoming
1980-1982
Postdoctoral Fellow, Los Alamos Scientific Laboratory
1980
Ph.D., in Physical-Analytical Chemistry, University of Utah
1976
B.S. in Chemistry and Mathematics, Northern Illinois University

Selected Awards

2013
ANACHEM Award
2013
Robert Boyle Prize for Analytical Science
2012
Fellow, Royal Society of Chemistry
2012
Fellow, American Association for the Advancement of Science
2012
Chemistry Department Alumni Award, University of Utah
2010-2012
Inaugural class, College of Reviewers, National Institutes of Health
2009
Department of Chemistry Distinguished Alumni Award, Northern Illinois University
2003
ACS Award in Spectrochemical Analysis

Research Interests

The Human Genome Project was the most ambitious research project in the history of analytical chemistry. The goal of that project was the determination of the primary structure of the human genome, which is a group of molecules with a combined molecular weight of 3 billion kilograms. Capillary array electrophoresis with laser induced fluorescence detection in a sheath flow cuvette was the primary analytical instrument used in sequencing the genome; that instrumentation was developed, in part, by the Dovichi research group.

Now that the genome is sequenced, interest is focused on the study of the proteome, which is the protein content of an organism, tissue, or cell. The Dovichi group is developing tools to study the proteome with two-dimensional capillary electrophoresis, coupled to either laser-induced fluorescence or mass spectrometry for detection. The group's long-term goal is to study protein expression in single cells and to determine how protein expression changes across a cellular population during cancer progression and during the development of an embryo. In the shorter term, we are developing tools to characterize post-translational modifications of proteins and to speed digestion and analysis of proteins.

Proteomics provides a parts list for a cell; it does not necessarily describe how those parts function. Ultimately, the characterization of a cells metabolism, which describes the production and consumption of small molecules, is necessary to understand health and disease. The Dovichi group also is studying metabolic pathways in single cells. In general, a fluorescent substrate is synthesized. Any enzymatic transformation of the substrate can be monitored with exquisite sensitivity as long as the fluorescent label is preserved. We are collaborating with Ole Hindsgaul and Monica Palcic of the Carlsberg Institute and Ron Schnaar at Johns Hopkins University to characterize sphingolipid metabolism in single cells. These glycolipids make up a very large fraction of neuronal membrane, and defects in their metabolism leads to devastating genetic diseases.

Recent Papers

  • Larrain, J.; Edwards, G.; Lee-Liu, D.; Mendez, E.; Penailillo, J.; Munoz, R.; Guzman, D.; Sun, L.; De Domenico, E.; Cebrian-Silla, A.; Dovichi, N.; Gilchrist, M.; Manuel Garcia-Verdugo, J.; Faunes, F. "Cellular and Molecular Mechanism of Spinal Cord Regeneration in the Frog Xenopus laevis." Mech. Dev. 2017, 145, S49-S49.
  • Zhang, Z.; Peuchen, E. H.; Dovichi, N. J. "Surface-Confined Aqueous Reversible Addition-Fragmentation Chain Transfer (SCARAFT) Polymerization Method for Preparation of Coated Capillary Leads to over 10,000 Peptides Identified from 25 ng HeLa Digest by Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry." Anal. Chem. 2017, 89, 6774-6780.
  • Sarver, S. A.; Schiavone, N. M.; Arceo, J.; Peuchen, E. H.; Zhang, Z.; Sung, L.; Dovichi, N. J. "Capillary electrophoresis coupled to negative mode electrospray ionization mass spectrometry using an electrokinetically-pumped nanospray interface with primary amines grafted to the interior of a glass emitter." Talanta 2017, 165, 522-525.
  • Zhang, Z.; Zhu, G.; Peuchen, E. H.; Dovichi, N. J. "Preparation of linear polyacrylamide coating and strong cationic exchange hybrid monolith in a single capillary, and its application as an automated platform for bottom-up proteomics by capillary electrophoresis-mass spectrometry." Microchimica Acta 2017, 184, 921-925.
  • Peuchen, E. H.; Zhu, G.; Sun, L.; Dovichi, N. J. "Evaluation of a commercial electro-kinetically pumped sheath-flow nanospray interface coupled to an automated capillary zone electrophoresis system." Analytical and Bioanalytical Chemistry 2017, 409, 1789-1795.
  • Krokhin, O. V.; Anderson, G.; Spicer, V.; Sun, L.; Dovichi, N. J. "Predicting Electrophoretic Mobility of Tryptic Peptides for High-Throughput CZE-MS Analysis." Anal. Chem. 2017, 89, 2000-2008.

Contact Information

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Research Specialties