Dr. Zhang obtained his B.S. degree in Inorganic Nonmetal Material Engineering from Central South University and Ph.D. in analytical chemistry from Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Subsequently, he conducted postdoctoral research at the University of Notre Dame. He joined the faculty at Notre Dame in 2016.
My research interest is to improve the identification performance of capillary zone electrophoresis (CZE) in mass spectrometry based proteomics analysis. Currently, reversed-phase liquid chromatography (RPLC) is the dominant separation method in proteomics analysis. However, CZE outperforms RPLC when dealing with less than tens of nanogram amounts of samples, and it has great potential in ultrasensitive and high throughput proteomics analysis. To overcome the disadvantages of CZE, such as a high concentration detection limit and low sample loading capacity, a solid phase extraction method based on strong cation exchange (SCX) monolith was developed for online sample preconcentration prior to CZE analysis. Moreover, the SCX monolith can be used as a microreactor for online sample preparation to minimize the sample lost and to improve the efficiency.
The developed microreactor-based platform has great potential for single cell proteomics analysis. Currently, a single blastomere of Xenopus embryo is used as a model. A single blastomere has hundreds of nanogram to microgram amounts of proteins depending on embryo stage, and they are significantly different starting from the 8-cell stage. More importantly, the single cell proteomic work on those blastomeres from the same and different stages will be invaluable for understanding blastomere lineage. In terms of sample preparation, we will inject the single cell into the SCX monolith, then do online lysis, reduction and digestion. The sample loss will be decreased to the minimum because the sample was retained on the SCX monolith during all the sample preparation steps. After online digestion, the peptides can be eluted by using single or pH gradient elution for CE-MS or LC-MS analysis.
- Zhang, Z., Qu, Y., Dovichi, N.J. "Capillary zone electrophoresis-mass spectrometry for bottom-up proteomics" 2018 TrAC - Trends in Analytical Chemistry, 108, pp. 23-37. DOI: 10.1016/j.trac.2018.08.008
- Qu, Y., Sun, L., Zhu, G., Zhang, Z., Peuchen, E.H., Dovichi, N.J. "Sensitive and fast characterization of site-specific protein glycosylation with capillary electrophoresis coupled to mass spectrometry" 2018 Talanta, 179, pp. 22-27. DOI: 10.1016/j.talanta.2017.10.015
- Zhang, Z., Dovichi, N.J. "Optimization of mass spectrometric parameters improve the identification performance of capillary zone electrophoresis for single-shot bottom-up proteomics analysis" 2018 Analytica Chimica Acta, 1001, pp. 93-99. DOI: 10.1016/j.aca.2017.11.023
- Qu, Y., Sun, L., Zhang, Z., Dovichi, N.J. "Site-Specific Glycan Heterogeneity Characterization by Hydrophilic Interaction Liquid Chromatography Solid-Phase Extraction, Reversed-Phase Liquid Chromatography Fractionation, and Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry" 2018 Analytical Chemistry, 90 (2), pp. 1223-1233. DOI: 10.1021/acs.analchem.7b03912
- Zhang, Z., Hebert, A.S., Westphall, M.S., Qu, Y., Coon, J.J., Dovichi, N.J. "Production of over 27 000 Peptide and Nearly 4400 Protein Identifications by Single-Shot Capillary-Zone Electrophoresis-Mass Spectrometry via Combination of a Very-Low-Electroosmosis Coated Capillary, a Third-Generation Electrokinetically-Pumped Sheath-Flow Nanospray Interface, an Orbitrap Fusion Lumos Tribrid Mass Spectrometer, and an Advanced-Peak-Determination Algorithm" 2018 Analytical Chemistry, Article in Press. DOI: 10.1021/acs.analchem.8b02991
- Boley, D.A., Zhang, Z., Dovichi, N.J. "Multisegment injections improve peptide identification rates in capillary zone electrophoresis-based bottom-up proteomics" 2017 Journal of Chromatography A, 1523, pp. 123-126. DOI: 10.1016/j.chroma.2017.07.022
For a full list of publications, please click here.