Rashna Balsara

Rashna Balsara

Biography

2014-present
Research Associate Professor, University of Notre Dame
2007-2014
Research Assistant Professor, University of Notre Dame
2005-2007
Walther Cancer Research Fellow, University of Notre Dame
2002-2005
Postdoctoral Fellow, University of Notre Dame
2001-2002
NIH Training Fellow, University of Illinois Urbana-Champaign
1998-2000
Postdoctoral Fellow, University of Illinois Urbana-Champaign
1998
Ph.D. in Molecular Biology, Bombay University
1991
M.S. in Molecular Biology, Bombay University
1985
B.S. in Microbiology, Bombay University

Selected Awards

1994
Young Scientist Fellowship
1991-1996
Council of Scientific and Industrial Research Fellowship, Government of India

Research Interests

Angiogenesis is an important process of forming new vessels that occurs in both physiological and pathophysiological settings. This process is a multistep event involving several proteins that function in a spatially and temporally controlled microenvironment. Components of the fibrinolytic pathway, namely plasminogen (Pg), urokinase plasminogen activator (uPA), its receptor (uPAR), tissue-type plasminogen activator (tPA), and the physiological inhibitor plasminogen activator inhibitor-1 (PAI-1) are some of the key players involved in angiogenic events.  The process of angiogenesis is initiated by activation of the normally quiescent endothelial cells, degradation of the extracellular matrix, followed by cellular proliferation, migration, adhesion, and finally formation of capillary sprouts.  Discerning the involvement of the individual fibrinolytic proteins in angiogenesis has been facilitated by utilizing primary endothelial cells obtained from various knockout mice (PAI-1-/-, uPA-/-, uPAR-/-, tPA-/-).

The fibrinolytic proteins are also known to transduce cell signaling by activation of tyrosine kinase receptors, such as, VEGR1 and VEGFR-2 to promote cell survival. Our studies have implicated a novel role of PAI-1 in modulating the Akt, and the JAK/STAT pathway thereby affecting endothelial apoptosis and cell cycle progression. Current studies involve elucidating the role of uPAR in endothelial cell adhesion and its ability to affect cell morphology. Organization of F-actin fibers, and cellular localization of phosphorylated Focal Adhesion Kinase (FAK) and vincullin are dramatically different in uPAR-/- endothelial cells compared to the WT cells. Furthermore, the ability of the receptor to modulate changes in cell morphology and adhesion is dependent on uPAR-mediated cell binding to the matrix via regulation of integrin signaling.

Therefore, the primary focus of interest is to study the role of fibrinolytic pathway components in events associated with angiogenesis, as well as to provide mechanisms by evaluating the underlying signal transduction pathways. The angiogenic study involves cell-based assays, molecular biology, and recombinant protein work, as well as utilizing cutting edge technology, such as, confocal microscopy and real-time microscopy.

Recent Papers

  • Fields, F.R., Carothers, K.E., Balsara, R.D., Ploplis, V.A., Castellino, F.J., Lee, S.W. "Rational design of syn-safencin, a novel linear antimicrobial peptide derived from the circular bacteriocin safencin AS-48" 2018 Journal of Antibiotics, 71 (6), pp. 592-600. DOI: 10.1038/s41429-018-0032-4
  • Holmes, A., Zhou, N., Donahue, D.L., Balsara, R., Castellino, F.J. "A deficiency of the GluN2C subunit of the N-methyl-D-aspartate receptor is neuroprotective in a mouse model of ischemic stroke" 2018 Biochemical and Biophysical Research Communications, 495 (1), pp. 136-144. DOI: 10.1016/j.bbrc.2017.10.171
  • Qiu, C., Yuan, Y., Zajicek, J., Liang, Z., Balsara, R.D., Brito-Robionson, T., Lee, S.W., Ploplis, V.A., Castellino, F.J. "Contributions of different modules of the plasminogen-binding Streptococcus pyogenes M-protein that mediate its functional dimerization" 2018 Journal of Structural Biology, Article in Press. DOI: 10.1016/j.jsb.2018.07.017
  • Yuan, Y., Balsara, R.D., Zajicek, J., Kunda, S., Castellino, F.J. "Discerning the Role of the Hydroxyproline Residue in the Structure of Conantokin Rl-B and Its Role in GluN2B Subunit-Selective Antagonistic Activity toward N-Methyl-d-Aspartate Receptors" 2016 Biochemistry, 55 (51), pp. 7112-7122. DOI: 10.1021/acs.biochem.6b00962
  • Johnson, J.J., Miller, D.L., Jiang, R., Liu, Y., Shi, Z., Tarwater, L., Williams, R., Balsara, R., Sauter, E.R., Stack, M.S. "Protease-activated Receptor-2 (PAR-2)-mediated Nf-κB activation suppresses inflammation-associated tumor suppressor MicroRNAs in oral squamous cell carcinoma" 2016 Journal of Biological Chemistry, 291 (13), pp. 6936-6945. DOI: 10.1074/jbc.M115.692640​​​​​​​
  • Cheriyan, J., Balsara, R.D., Hansen, K.B., Castellino, F.J. "Pharmacology of triheteromeric N-Methyl-d-Aspartate Receptors" 2016 Neuroscience Letters, 617, pp. 240-246. DOI: 10.1016/j.neulet.2016.02.032

Contact Information

Primary Research Areas