NIH funding for CD spectropolarimeter and stopped-flow apparatus

CD spectroscopy exploits the chiral properties of small molecules, proteins and nucleic acids to provide information on molecular structure and its changes.  For example, changes in protein secondary structure caused by unfolding (induced by perturbants such as temperature or pH), or folding (induced by the binding of a small molecule ligand, protein, or nucleic acid) can be quickly and easily quantified as changes in the far-UV CD spectrum.  Separately, CD spectroscopy is also an essential technique to study chiral-selective properties of small molecules, including drugs, fluorescent dyes, and chemical catalysts designed to mimic enzymatic catalysis.  This new instrument will permit simultaneous fluorescence detection, and seamless integration with the new stopped-flow kinetic device, further enhancing the utility of CD spectroscopy for biomedical studies.