CRISPR technology is best known as a gene-editing tool. Cas12a has been harnessed for genome editing for its ability to generate double-strand DNA (dsDNA) breaks and indiscriminate single-strand DNA (ssDNA). In a recent publication, English et al developed a group of stimuli-responsive hydrogels to respond to programmable nuclease Cas12a. This seminar will discuss the programmability of Cas enzymes to improve hydrogel systems allowing for a range of in vitro applications.
English et al. Programmable CRISPR-responsive smart materials. Science (2019)