The discovery of RNA guided endonucleases has become one of the most important tools for gene edition and biotechnology. The applications of this technology in mammalian cells has resulted in a growing field as not only an easy programmable nuclease for gene edition, but also use as a regulator of gene expression, an epigenetic modulator, and as an imaging agent. This seminar highlights recent work conducted using supramolecular chemistry to bypass the many challenges of plasmid transfection of CRISPR/Cas9 systems, which include undesired permanent DNA recombination as well as immunogenic responses. This novel strategy for the direct delivery of Cas9 using a penetrating peptide vehicle further encourages the search and development of synthetic systems for transitory endonucleases delivery.
Lostalé-Seijo, I., Louzao, I., Juanes, M., Montenegro, J., (2017). Peptide/Cas9 nanostructures for ribonucleoprotein cell membrane transport and gene edition. Chemical Science, 8(12), 7923-7931. DOI: 10.1039/c7sc03918b