Imaging Dynamic Events Inside Living Cells
Abstract: Living cells carry out countless chemical reactions regulated by a variety of environmental parameters; concentration, diffusion, redox state, pH, and active transport. The goal of research in the Payne Lab is to understand the mechanism of intracellular reactions in relation to the cellular environment. Current research focuses on two aspects of cellular regulation; spatial localization of enzymes in vesicles and diffusion within the crowded environment of the cytosol. The highly heterogeneous environment of the cell requires a technique that can measure both the “where” and “when” of the event of interest. Video-rate fluorescence microscopy provides this information, but suffers from limitations associated with time-resolved imaging in the noisy environment of live cells. For this reason, we are simultaneously pursuing new approaches to the intracellular targeting of bright fluorophores and the development of new fluorescence microscopy systems for time-resolved cellular imaging. This seminar will focus on a specific example illustrating the role of spatially localized enzymes in the degradation of low-density lipoprotein (LDL) in living cells as well as the fluorescence microscopy techniques being developed in the Payne Lab.