Subhash Basu

Subhash Basu

Emeritus Professor

Biography

Professor Basu received his B.S. in 1958 and M.S. in 1960, both from Calcutta University, and his Ph.D. in 1966 from the University of Michigan. He was a postdoctoral fellow, 1966-67, and a Helen Hay Whitney Research Fellow, 1967-70, at Johns Hopkins University before joining the faculty at Notre Dame in 1970. In 1976, he received a Doctorate of Science (D.Sc.) from Calcutta University. Honors received: Jacob Javits Neuroscience Research Award (NIH); NSF-CSIR TOKTEN Visiting Fellow to India; Elected Fellow of AAAS; Induced Member of The Johns Hopkins Honor Society; Editor of Glycoconjugate Journal and Current Drug Targets.

Research Interests

The research interests of Professor Subhash Basu's group focus on two major areas: the role of sphingolipids during apoptosis and metastasis of cancer cells and the role of DNA "Replisome complex" in developing brain tissues and apoptotic carcinoma cells.

 

Transcriptional and Post-translational Regulation of Glycosyltransferases: Previous work from Dr. Basu's laboratory established the in vitro biosynthetic pathways for GD1a ganglioside (neuronal cell surface glycolipid) and SALeX (cancer cell surface glycolipid) in developing brain tissues and metastatic cancer cells. Enzymatic characterization, purification and the cloning of twelve different glycolipid: glycosyltransferases (GSL: GLTs) are continuing projects of his group. Studies on the transcriptional and the post-translational regulation of at least five crucial enzymes (GlcT-1, GalT-2, GalT-4, SAT-2, and SAT-3) which are involved in the pathways (for biosynthesis of SA-LeX) in the human carcinoma cells during apoptosis induced by anti-cancer agents (cis-platin or cis-DDP, L-PPMP, Tamoxifen, Betulinic acid, etc.) are of present interest in his group. Involvement of sphingolipids in the mitochondrial siganaling pathway (MSP) and activation of caspaseshas been studied by Basu's group recently and further studies on its mechanism are in progress.

DNA Replication and Regulation: During neuronal cell development (in ECB, embryonic chicken brains) and in apoptosis of human carcinoma cells (breast, SKBR3 and colon, Colo-205), DNA replication is regulated by "Replisome complexes" containing DNA polymerase-alpha, helicase-III, DPAL, primase and many more yet unidentified gene products. Some of those components of the "Replisome Complexes" have been identified by Basu's group. Using 2-D NMR studies, Basu's group has established that during apoptosis of carcinoma cells by cis-platin, DNA biosynthesis is inhibited by binding of Pt (II) to the Zinc-binding domain (containing four cysteines) of the DNA polymerase-alpha. This reaction is several orders faster than the cis-DDP (cis-diaminodichloro platinum/II) binding to the N-7 positions of guanine in the DNA chains. Further studies by 2D NMR on the binding specificity of the PT(II) to the eukaryotic DNA polymerase-alpha are in progress. The destabilization of the Replisome complexes during apoptosis of the carcinoma cells by anti-cancer agents is the present focus of Basu's group._ Apoptotic signaling mechanism of cis-platin (cis-DDP) by a non- MSP (mitochondrial signaling pathway) with caspase-3 activation is under study by Basu's group.

Recent Papers

  • "Detection of Biological Materials by Gold Nano-Biosensor-Based Electrochemical Method" J. Jiang, M. Basu, S. Seggerson, A. Miller, M. Pugia, and S. Basu, in Nano Technologies for Life Sciences: 8th volume, Nanotechnologies for Biosensors (editor, Dr. C. Kumar); Journal of Biomedical Nanotechnology, pp. 208-239 (2007).
  • "Immunological Evaluation of Urinary Trypsin Inhibitors in Blood and Urine: Role of N- &O-)-Linked Glycoproteins" M. Pugia, S. A. Jortani, M. Basu, R. Sommer, H-H. Kuo, S. Murphy, D. James Vranish, P. Boyle, D. Budzinski, R. Valdes, Jr, and S. Basu. Glycoconjugate J., 24,5-15 (2007).
  • "Apoptosis of human breast carcinoma cells in the presence of cis-platin and L-/D-PPMP: IV. Modulation of Replication Complexes and Glycolipid: Glycosyltransferases,".P. J. Boyle, R. Ma, N. Tuteja, S. Banerjee, and S. Basu, Glycoconjugate J. 23, 175-187 (2006).
  • "Glycosphingolipid Metabolism and Signaling in Apoptotic Cancer Cells." S. Basu, R. Ma, M. Basu, H. Goodson, B. Smith, and S. Banerjee, Sphingolipid Metabolizing Enzymes (editors Drs. D. K. Haldar and Dr. S. K. Das) Research Signpost, pp. 81-100, (2004).
  • "Regulation of Glycosyltransferase Genes in Apoptotic Breast Cancer Cells by Inhibitors of Glycolipid and DNA Biosynthesis," Rui Ma, Elizabeth A. Hopp, N. Mathew Decker, Audrey J Loucks, James R. H. Johnson, Joseph R. Moskal, Manju Basu, Sipra Banerjee, and Subhash Basu, Symposium Volume published for MICC-III held in Taiwan on August 9-12, 2007 (editor: Albert Wu; Kluwer Academic /Plenum Publisher; New York; 2009)
  • "Post-Translational and Transcriptional Regulation of Glycolipid Glycosyltransferase Genes in Apoptotic Breast Carcinoma Cells: VII. After Treatment with L-PPMP," Rui Ma, N. Matthew Decker, Vesta Anilus, Joseph R. Moskal, Joseph Bergdorf, James Johnson, Manju Basu, Sipra Banerjee, and Subhash Basu, Glycoconjugate J. 26, pp. 647-661 (2009)

Contact Information

  • Emeritus Professor
  • Office: 342A Nieuwland Science
  • Phone: 574-631-5759
  • Send an email