Matthew Champion
Associate Professor
- Office
- 140B Mccourtney Hall
Notre Dame, IN 46556 - Phone
- +1 574-631-1787
- mchampio@nd.edu
Research Areas
- Biochemistry
- Physical/Analytical Chemistry
Research Specialties
- Life Processes
- Measurement
Prospective Graduate Students
Biography
| Year | Title |
|---|---|
| 2019-present | Associate Professor, University of Notre Dame |
| 2015-2019 | Research Associate Professor, University of Notre Dame |
| 2009-2015 | Research Assistant Professor, University of Notre Dame |
| 2003-2009 | Applied Biosystems (AB Sciex), Mass-Spectrometry-Proteomics Group |
| 2005 | Ph.D. in Biochemistry, Texas A&M University |
| 1997 | B.S. in Microbiology, University of Iowa |
Research Interests
The Champion group is primarily interested in developing and exploiting novel approaches to identify and characterize the components of secreted proteins from virulent microorganisms. We heavily utilize the 'awesome power of genetics' coupled with state-of-the art quantitative proteomics to enrich, identify and quantify the proteins responsible for biological phenotypes. We have ongoing projects in pathogenic mycobacteria, protein translation in E. coli through the PTRN, and quantitative protein secretion measured using capillary electrophoresis. One example is described below.
Mycobacteria, the causative agent of tuberculosis, like most organisms secretes proteins for biochemical functions. Secreted proteins are a proteome of 'target'-enriched material which contains virulence-factors, antigenic-determinants, and targets for treatment/detection. EsxA and EsxB (ESAT-6 and CFP10) are essential virulence factors exported by mycobacteria and other Gram positive pathogens. Identification of proteins that are required for effective synthesis and secretion of these crucial virulence determinants has been hindered by a lack of saturating genetic screens; high-throughput biochemical detection, and accurate quantification of disease phenotypes. We designed orthogonal antibody-free assays using a slew of proteogenetic approaches to comprehensively dissect the individual contribution of gene products towards a functioning disease secretion system (ESX-1) in pathogenic mycobacterium. This framework is highly extensible to the analysis of other pathogen and protein secretion systems, and has uncovered novel genes, pathways, and crosstalk among secretion systems.
Selected Publications
- Sterling, F. R.; D'Amico, J.; Brumfield, A. M.; Huegel, K. L.; Vaughan, P. S.; Morris, K.; Schwarz, S.; Joyce, M. V.; Boggess, B.; Champion, M. M.; Maciuba, K.; Allen, P.; Marasco, E.; Koch, G.; Gonzalez, P.; Hodges, S.; Leahy, S.; Gerstbauer, E.; Hinchcliffe, E. H. and Vaughan, K. T. "StARD9 is a Novel Lysosomal Kinesin Required for Membrane Tubulation, Cholesterol Transport and Purkinje Cell Survival" 2023 Journal of Cell Science, 136 (5), jcs260662. DOI: 10.1242/jcs.260662.
- Weaver, S. D.; DeRosa, C. M.; Schultz, S. R. and Champion, M. M. "PrIntMap-R: An Online Application for Intraprotein Intensity and Peptide Visualization from Bottom-Up Proteomics" 2023 Journal of Proteome Research, 22 (2), pp. 432-441. DOI: 10.1021/acs.jproteome.2c00606.
- Avila-Cobian, L. F.; De Benedetti, S.; Kim, C.; Feltzer, R.; Champion, M. M.; Fisher, J. F. and Mobashery, S. "In Vitro Studies of the Protein-Interaction Network of Cell-Wall Lytic Transglycosylase RlpA of Pseudomonas Aeruginosa" 2022 Communications Biology, 5 (1), 1314. DOI: 10.1038/s42003-022-04230-x.
- Saelens, J. W.; Sweeney, M. I.; Viswanathan, G.; Xet-Mull, A. M.; Smith, K.; Sisk, D. M.; Hu, D. D.; Cronin, R. M.; Hughes, E. J.; Brewer, W. J.; Coers, J.; Champion, M. M.; Champion, P. A.; Lowe, C. B.; Smith, C. M.; Lee, S.; Stout, J. E. and Tobin, D. M. "An Ancestral Mycobacterial Effector Promotes Dissemination of Infection" 2022 Cell, 185 (24), pp.4507-4525. DOI: 10.1016/j.cell.2022.10.019.
- Mousseau, C. B.; Pierre, C. A.; Hu, D. D. and Champion, M. M. "Miniprep Assisted Proteomics (MAP) for Rapid Proteomics Sample Preparation" 2023 Analytical Methods, 15 (7), pp.916-924. DOI: 10.1039/d2ay01549h.
- Cronin, R. M.; Ferrell, M. J.; Cahir, C. W.; Champion, M. M. and Champion, P. A. "Proteo-Genetic Analysis Reveals Clear Hierarchy of ESX-1 Secretion in Mycobacterium Marinum" 2022 Proceedings of the National Academy of Sciences of the United States of America, 119 (24), e2123100119. DOI: 10.1073/pnas.2123100119.