Anthony S. Serianni

Anthony S. Serianni

Biomolecular Structure and NMR Spectroscopy


Professor, University of Notre Dame
Associate Professor, University of Notre Dame
Assistant Professor, University of Notre Dame
Postdoctoral Research Associate, Cornell University
Ph.D. in Biochemistry, Michigan State University
B.S. in Biochemistry, Albright College

Selected Awards

Elected Fellow, Royal Society of Chemistry
Elected Fellow, American Chemical Society
Elected Fellow, American Association for the Advancement of Science
Melville L. Wolfrom Award in Carbohydrate Chemistry, ACS Division of Carbohydrate Chemistry
Kaneb Teaching Award
John A. Boezi Memorial Alumnus Award, Michigan State University

Research Interests

Professor Serianni's research focuses on the use of stable isotopically-labeled compounds to examine structure, conformation and reactivity of carbohydrates and nucleic acids in solution using modern multidimensional NMR methods and computational techniques. His group is interested in developing and improving chemical and chemi-enzymic methods to prepare carbohydrate-containing biomolecules containing one or more sites of isotopic enrichment, with 13C, 2H and 15N of primary concern. NMR studies of these labeled biomolecules permit a more detailed assessment of their 3-D structures in solution, information which is critical to understanding the fundamental factors controlling molecular recognition in biological processes.

Of current interest is the use of stable isotopes in the assessment of DNA and RNA structure in solution. Research is under way to: a) develop efficient chemical, chemi-enzymic, and/or biological methods to introduce isotopes selectively or uniformly into nucleosides and oligonucleotides, b) apply 2-D and 3-D NMR methods to decipher the NMR spectra of labeled oligonucleotides, and c) assess the merits of 13C-1H and 13C-13C spin-coupling constants within the furanose rings of RNA and DNA as structural probes. NMR data are complemented and extended by ab initio molecular orbital calculations which can assess the energetics and structural features of furanose ring pseudorotation and predict 13C-1H and 13C-13C spin-couplings for various pathways in these rings, and by X-ray crystallography. The long range objective is to apply this information to NMR solution studies of CCAAT-containing oligonucleotides involved in the NF-1 recognition site at the origin of replication of human adenovirus, and other biologically-important DNA and RNA oligomers.

Other work involves studies of carbohydrate metabolism via in vivo 13C and 19F NMR spectroscopy, and the development of automated devices for chemical and biological synthesis of labeled biomolecules.

Recent Papers

  • Klepach, T.; Zhao, H.; Hu, X.; Zhang, W.; Stenutz, R.; Hadad, M.J.; Carmichael, I.; Serianni, A.S. "Informing saccharide structural NMR studies with density functional theory calculations." Methods in Molecular Biology 2015, 1273, 289-331.
  • Larson, D.J.; Middle, L.; Vu, H.; Zhang, W.H.; Serianni, A.S.; Duman, J.; Barnes, B.M. "Wood frog adaptations to overwintering in Alaska: new limits to freezing tolerance." J. Exp. Biol. 2014, 217(12), 2193-2200.
  • Zhang, W.H.; Oliver, A.G.; Vu, H.M.; Duman, J.G.; Serianni, A.S. "Methyl 4-O-beta-D-xylopyranosyl beta-D-mannopyranoside, a core disaccharide of an antifreeze glycolipid." Acta Crystallogr. C 2013, 69(9), 1047.
  • Zhang, W.H.; Hu, X.S.; Carmichael, I.; Serianni, A.S. "Methyl [C-13]Glucopyranosiduronic Acids: Effect of COOH Ionization and Exocyclic Structure on NMR Spin-Couplings." J. Org. Chem. 2012, 77(21), 9521-9534.
  • Zhang, W.H.; Serianni, A.S. "Phosphate-Catalyzed Degradation of D-Glucosone in Aqueous Solution is Accompanied by C1-C2 Transposition." J. Am. Chem. Soc. 2012, 134(28), 11511-11524.

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