- Director of Research, Advanced Diagnostics and Therapeutics, University of Notre Dame
- Concurrent Professor, Department of Chemistry and Biochemistry, University of Notre Dame
- Research Chemist, ERDC-CERL, Army Corp of Engineers
- Associate Professor of Chemistry, West Virginia University
- Assistant Professor of Chemistry, West Virginia University
- Postdoctoral Researcher, University of Washington
- Postdoctoral Researcher, University of South Florida
- Ph.D. in Analytical Chemistry, University of Illinois
- B.S. in Chemistry, Saint Louis University
We believe new analytical measurement tools play a critical role in overcoming the grand challenges presented by the complexity of living systems. The wide range of projects in our lab involves microfluidics, chemical separations, proteomics, and a variety of other tools and methods. We approach research in a team oriented manner and work with collaborators at Notre Dame and other institutions.
A great need exists for the rapid and accurate detection of bacterial and viral pathogens in both clinical and field settings. For medical diagnostics, most gold-standard methods for detection and identification of harmful bacteria take days to produce results. Moreover, adequate sensing for biological warfare agents for both homeland defense and protection of military personnel do not currently exist. Current portable systems rely on selective molecular recognition and are only capable of detecting a relatively limited number of pathogens. In response to these problems, we are working on a novel approach centered on a microfluidic system that augments the selective recognition with physical characterization of the pathogens. Our research is addressing the questions: how many physical parameters can we characterize? With what precision can we characterize these parameters? What improvement in selectivity is gained? Can we differentiate viable and non-viable pathogens?
There is also a great need for miniature and portable separation systems that can improve the selectivity of sensors. To this aim, we have invented a new microfluidic separation method called traveling wave electrophoresis (TWE). This separation method rivals capillary electrophoresis in performance and has many unique features, including the ability to switch between separative and non-separative transport by simply tuning the frequency. With low voltage (+/- 0.5 V) and power consumption, it will be possible to power and control these devices with cell phones. Currently, the mechanisms of the TWE transport and separation processes are not fully understood. To improve this understanding, we are collaborating with Boyd Edwards from Utah State University, whose team models the separation process. Experimentally, we are focusing on protein and nucleic acid separations, and on creating improved architectures and microfabrication methods.
- Wang,H.; Nandigana, V.R.R.; Jo, K.D.; Aluru, N.R.; Timperman, A.T. "Controlling the ionic current rectification factor of a nanofluidic/microfluidic interface with symmetric nanocapillary interconnects." Anal. Chem. 2015, 87, 3598-3605.
- Grimme, J.; King, T.; Jo, K.D.; Cropek, D.; Timperman, A.T. "Development of fieldable lab-on-a-chip systems for detection of a broad array of targets from toxicants to biowarfare agents." J. Nanotech. Eng. Med. 2013, 4, 020904-1 - 020904-8.
- Jo, K.D.; Schiffbauer, J.E.; Edwards, B.E.; Carroll, R.L.; Timperman, A.T. "Fabrication and performance of a microfluidic traveling-wave electrophoresis system." Analyst 2012, 137, 875-883.
- Reschke, B.R.; Timperman, A.T. "A study of electrospray ionization emitters with differing geometries with respect to flow rate and electrospray voltage." J. Am. Soc. Mass Spectr. 2011, 22, 2115-2124.
- Mao, X.; Reschke, B.R.; Timperman, A.T. "Analyte transport past a nanofluidic intermediate electrode junction in a microfluidic device." Electrophoresis 2010, 31, 2686-2694.